Journal:

Article Title: The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos 1 [w]

doi: 10.1104/pp.015214

Figure Lengend Snippet: Quality control of microarray experiments

Article Snippet: The purified DNA was denatured in 50% (w/v) dimethyl sulfoxide and spotted in four replicates onto Corning microarray technology-gamma amino propyl silane aminosilane-coated glass microscope slides (Corning, NY) by a 417 Arrayer (Affymetrix, Woburn, MA).

Techniques: Microarray

Journal: PLoS ONE

Article Title: Efficient Generation of Myostatin (MSTN) Biallelic Mutations in Cattle Using Zinc Finger Nucleases

doi: 10.1371/journal.pone.0095225

Figure Lengend Snippet: We also produced a healthy cloned calf with monoallelic MSTN mutation, which consisted of a 55-bp insertion in intron 1, and caused the premature termination of translation. This allele is represented by “55 bp inserted” in the chart, and “6 bp/117 bp deleted” represents the double-muscled cloned bovine previously discussed in . The level of MSTN protein with a functional C-terminal domain was reduced by approximately 50% in both mutant calves, compared to that of the WT calf. The ELISA data were analyzed using paired Student’s t -tests. The error bars represent the standard deviations of three experiments (* P <0.05 indicates a statistically significant difference compared to the WT calf).

Article Snippet: The amount of MSTN protein with a C-terminal domain was determined using an ELISA kit (Life Sciences Advanced Technologies, St. Petersburg, FL, USA), according to the manufacturer’s protocol.

Techniques: Produced, Clone Assay, Mutagenesis, Functional Assay, Enzyme-linked Immunosorbent Assay