Journal: Science Advances

Article Title: Programmable low-cost DNA-based platform for viral RNA detection

doi: 10.1126/sciadv.abc6246

Figure Lengend Snippet: ( A ) Schematic of the DNA nanoswitch and detection of a viral RNA sequence. nt, nucleotide. ( B ) Fast development cycle of nanoswitches for RNA viruses. ( C ) Nanoswitch-based assay allows direct detection using a nonenzymatic approach (top) and can optionally be combined with an isothermal amplification step like NASBA (nucleic acid sequence–based amplification) (bottom).

Article Snippet: For the NASBA-based detection of SARS-CoV-2 RNA purchased from Twist Biosciences, NASBA kits purchased from Life Sciences Advanced Technologies Inc. were used.

Techniques: Sequencing, Amplification

Journal: Science Advances

Article Title: Programmable low-cost DNA-based platform for viral RNA detection

doi: 10.1126/sciadv.abc6246

Figure Lengend Snippet: ( A ) Positive identification of ZIKV RNA in spiked urine by first isolating in vitro transcribed target RNA using a commercially available viral RNA extraction kit, followed by direct, nonenzymatic detection using DNA nanoswitches. ( B ) Positive identification of ZIKV RNA from virus particles spiked into urine based on NASBA. ( C ) Positive detection of in vitro transcribed SARS-CoV-2 RNA in human saliva based on NASBA. Error bars represent SD from triplicate experiments.

Article Snippet: For the NASBA-based detection of SARS-CoV-2 RNA purchased from Twist Biosciences, NASBA kits purchased from Life Sciences Advanced Technologies Inc. were used.

Techniques: In Vitro, RNA Extraction

Journal: Journal of Spine Surgery

Article Title: Surgical treatment of cervical radiculopathy using an anterior cervical endoscopic decompression

doi: 10.21037/jss.2019.09.24

Figure Lengend Snippet: Anterior endoscopic cervical discectomy and foraminoplasty. Endoscopic view of axilla of the exiting nerve root.

Article Snippet: The use of monosegmental anterior endoscopic discectomy to manage CR was described by Saringer ( 9 ) Choi ( 10 - 12 ) and Ruetten ( 13 ).

Techniques:

Journal: Journal of Spine Surgery

Article Title: Surgical treatment of cervical radiculopathy using an anterior cervical endoscopic decompression

doi: 10.21037/jss.2019.09.24

Figure Lengend Snippet: Cervical hematoma following ACED. ACED, anterior cervical endoscopic discectomy.

Article Snippet: The use of monosegmental anterior endoscopic discectomy to manage CR was described by Saringer ( 9 ) Choi ( 10 - 12 ) and Ruetten ( 13 ).

Techniques:

Journal: PLoS ONE

Article Title: Efficient Generation of Myostatin (MSTN) Biallelic Mutations in Cattle Using Zinc Finger Nucleases

doi: 10.1371/journal.pone.0095225

Figure Lengend Snippet: We also produced a healthy cloned calf with monoallelic MSTN mutation, which consisted of a 55-bp insertion in intron 1, and caused the premature termination of translation. This allele is represented by “55 bp inserted” in the chart, and “6 bp/117 bp deleted” represents the double-muscled cloned bovine previously discussed in . The level of MSTN protein with a functional C-terminal domain was reduced by approximately 50% in both mutant calves, compared to that of the WT calf. The ELISA data were analyzed using paired Student’s t -tests. The error bars represent the standard deviations of three experiments (* P <0.05 indicates a statistically significant difference compared to the WT calf).

Article Snippet: The amount of MSTN protein with a C-terminal domain was determined using an ELISA kit (Life Sciences Advanced Technologies, St. Petersburg, FL, USA), according to the manufacturer’s protocol.

Techniques: Produced, Clone Assay, Mutagenesis, Functional Assay, Enzyme-linked Immunosorbent Assay